A new electrochemical method for the determination of chondroitin sulfate based on its supramolecular interaction with cupferron-lead(II) complex

In this paper, the interaction of cupferron (Cup) and lead (II) complex [Cup-Pb (II)] with chondroitin sulfate (CS) was investigated by linear sweep voltammetric method. In the selected medium of pH 5.5 (acetic acid-hexamine buffer solution), Cup can interact with Pb (II) to form a stable complex of [Cup-Pb(II)], which has a sensitive second order derivative polarographic reductive peak at -0.64V (vs.SCE). After the addition of CS into Cup-Pb (II) complex solution, the reductive peak current decreased without any shift of the peak potential and no new peak appeared, which indicated that an unelectroactive supramolecular complex of CS with [Cup-Pb(II)] was formed. The binding reaction conditions were carefully investigated. Under the optimal conditions, the interaction mechanism was discussed. The decrease of reductive peak current was directly proportional to the CS concentration, thus a new quantitative determination method for CS was established with the linear regression equation as ΔIp″(nA)=36.97 C/mg L-1+12.45 (n=10, γ=0.995). The effects of other substances on the determination were carefully investigated and three synthetic samples were determined with satisfactory results. The binding constant (βs) and the binding number (m) of CS with [Cup-Pb(II)] complex were calculated from the voltammetric data with the results as βs=1.89×1010 and m≈2.5

Modeling the role of p53 pulses in DNA damage- induced cell death decision

<p>Abstract</p> <p>Background</p> <p>The tumor suppressor p53 plays pivotal roles in tumorigenesis suppression. Although oscillations of p53 have been extensively studied, the mechanism of p53 pulses and their physiological roles in DNA damage response remain unclear.</p> <p>Results</p> <p>To address these questions we presented an integrated model in which Ataxia-Telangiectasia Mutated (ATM) activation and p53 oscillation were incorporated with downstream apoptotic events, particularly the interplays between Bcl-2 family proteins. We first reproduced digital oscillation of p53 as the response of normal cells to DNA damage. Subsequent modeling in mutant cells showed that high basal DNA damage is a plausible cause for sustained p53 pulses observed in tumor cells. Further computational analyses indicated that p53-dependent PUMA accumulation and the PUMA-controlled Bax activation switch might play pivotal roles to count p53 pulses and thus decide the cell fate.</p> <p>Conclusion</p> <p>The high levels of basal DNA damage are responsible for generating sustained pulses of p53 in the tumor cells. Meanwhile, the Bax activation switch can count p53 pulses through PUMA accumulation and transfer it into death signal. Our modeling provides a plausible mechanism about how cells generate and orchestrate p53 pulses to tip the balance between survival and death.</p

Identification of Protein Pupylation Sites Using Bi-Profile Bayes Feature Extraction and Ensemble Learning

Pupylation, one of the most important posttranslational modifications of proteins, typically takes place when prokaryotic ubiquitin-like protein (Pup) is attached to specific lysine residues on a target protein. Identification of pupylation substrates and their corresponding sites will facilitate the understanding of the molecular mechanism of pupylation. Comparing with the labor-intensive and time-consuming experiment approaches, computational prediction of pupylation sites is much desirable for their convenience and fast speed. In this study, a new bioinformatics tool named EnsemblePup was developed that used an ensemble of support vector machine classifiers to predict pupylation sites. The highlight of EnsemblePup was to utilize the Bi-profile Bayes feature extraction as the encoding scheme. The performance of EnsemblePup was measured with a sensitivity of 79.49%, a specificity of 82.35%, an accuracy of 85.43%, and a Matthews correlation coefficient of 0.617 using the 5-fold cross validation on the training dataset. When compared with other existing methods on a benchmark dataset, the EnsemblePup provided better predictive performance, with a sensitivity of 80.00%, a specificity of 83.33%, an accuracy of 82.00%, and a Matthews correlation coefficient of 0.629. The experimental results suggested that EnsemblePup presented here might be useful to identify and annotate potential pupylation sites in proteins of interest. A web server for predicting pupylation sites was developed

A pupil-positioning method based on the starburst model

Human eye detection has become an area of interest in the field of computer vision with an extensive range of applications in human-computer interaction, disease diagnosis, and psychological and physiological studies. Gaze-tracking systems are an important research topic in the human-computer interaction field. As one of the core modules of the head-mounted gaze-tracking system, pupil positioning affects the accuracy and stability of the system. By tracking eye movements to better locate the center of the pupil, this paper proposes a method for pupil positioning based on the starburst model. The method uses vertical and horizontal coordinate integral projections in the rectangular region of the human eye for accurate positioning and applies a linear interpolation method that is based on a circular model to the reflections in the human eye. In this paper, we propose a method for detecting the feature points of the pupil edge based on the starburst model, which clusters feature points and uses the RANdom SAmple Consensus (RANSAC) algorithm to perform ellipse fitting of the pupil edge to accurately locate the pupil center. Our experimental results show that the algorithm has higher precision, higher efficiency and more robustness than other algorithms and excellent accuracy even when the image of the pupil is incomplete.Science and Technology Support Plan Project of Hebei Province (grant numbers 17210803D and 19273703D Science and Technology Spark Project of the Hebei Seismological Bureau (grant number DZ20180402056) Education Department of Hebei Province (grant number QN2018095) Polytechnic College of Hebei University of Science and Technolog

ZnCdS Dotted with Highly Dispersed Pt Supported on SiO2 Nanospheres Promoting Photocatalytic Hydrogen Evolution

[EN] The efficiency of solar hydrogen evolution closely depends on the fast transfer of charge carriers and the effective use of visible light. In this work, a novel photocatalyst SiO2/ZnCdS/Pt was successfully prepared to solve these two problems. An artistic structure of the photocatalyst was constructed and ZnCdS was successfully wrapped on the surface of SiO2 spheres with uniform Pt nanoparticles (NPs) in a size of 4.1 +/- 0.7 nm highly dispersed on the ZnCdS shell through the self-assembly method. Pt NPs can absorb the scattered light in the near field of SiO2 spheres. With the synergistic effect of SiO2 spheres and small highly dispersed Pt NPs, the absorption of visible light was significantly promoted. Meanwhile, the electron-hole recombination was also effectively inhibited, thus improving the photocatalytic activity. The hydrogen production activity of the highly efficient photocatalyst was as high as 8.3 mmol g(-1) h(-1) under visible light (lambda > 420 nm). The photocatalytic activity of SiO2/ZnCdS/Pt was 2.9 times higher than that of the ZnCdS/Pt photocatalyst.This work was supported by the National Natural Science Foundation of China (21976111), Shandong Provincial Natural Science Foundation (ZR2019MB052), and Large Instrument Open Foundation of Shandong Normal University (KFJJ2019004; KFJJ2021006).Liu, K.; Peng, L.; Zhen, P.; Chen, L.; Song, S.; García Gómez, H.; Sun, C. (2021). ZnCdS Dotted with Highly Dispersed Pt Supported on SiO2 Nanospheres Promoting Photocatalytic Hydrogen Evolution. The Journal of Physical Chemistry C. 125(27):14656-14665. https://doi.org/10.1021/acs.jpcc.1c0353514656146651252

Catalyst performance of novel Pt/Mg(Ga)(Al)O catalysts for alkane dehydrogenation

a b s t r a c t The dehydrogenation of ethane and propane using a Pt catalyst supported on a novel Mg(Ga)(Al)O mixed oxide support was investigated. Catalyst performance is strongly dependent on Ga content in the support, a peak in activity for both ethane and propane dehydrogenation occurs at Ga/Pt = 1.4-5.4, and selectivity is a monotonic function of Ga/Pt, reaching nearly 100% at Ga/Pt = 5.4. The addition of hydrogen to the feed resulted in a peak in activity with respect to H 2 /alkane. The increase in dehydrogenation rate with H 2 addition is attributed to H-atom-assisted dehydrogenation of alkyl species formed upon dissociative adsorption of the reactant alkane. Beyond the peak in activity with H 2 addition, a further increase in H 2 feed concentration contribute to alkene hydrogenation, thereby reducing the net rate of dehydrogenation. Hydrogen addition to the feed, however, had relatively little effect on alkene selectivity, which remained near 100%. The presence of Ga also suppressed coke formation. Interestingly, less coke was formed during propane dehydrogenation than ethane dehydrogenation, and no correlation was found between coke formation and catalyst deactivation. Thus, the extent of deactivation was lower for ethane than propane dehydrogenation, whereas the amount of coke deposited was higher in the former case. Since the amount of carbon deposited as coke is higher than the amount of exposed Pt, it is concluded that most of the coke resides on the support, and that only a small amount resides on the Pt particles. The higher level of deactivation seen during propane versus ethane dehydrogenation is attributed to a higher coverage of Pt by coke precursors derived from propane than ethane

Properties and Photocatalytic Activity of β

β-Ga2O3 nanorods are prepared by hydrothermal method and characterized by X-ray diffraction, high-resolution transmission electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, and photoluminescence spectra. The results reveal that high crystallinity, monoclinic phase of β-Ga2O3 nanorods were prepared with a diameter of about 60 nm and length of 500 nm. Photoluminescence study indicates that the β-Ga2O3 nanorods exhibit a broad blue light emission at room temperature. The β-Ga2O3 nanorods displayed high photocatalytic activity under simulated solar irradiation; after 2 h irradiation, over 95% of methylene blue solution and over 90% of methyl orange solution were decolorized. Since this process does not require additional hydrogen peroxide and uses solar light, it can be developed as an economically feasible and environmentally friendly method to treat dye effluent

Phloretin Prevents Diabetic Cardiomyopathy by Dissociating Keap1/Nrf2 Complex and Inhibiting Oxidative Stress

Hyperglycemia induces chronic inflammation and oxidative stress in cardiomyocyte, which are the main pathological changes of diabetic cardiomyopathy (DCM). Treatment aimed at these processes may be beneficial in DCM. Phloretin (PHL), a promising natural product, has many pharmacological activities, such as anti-inflammatory, anticancer, and anti-oxidative function. The aim of this study was to investigate whether PHL could ameliorate the high glucose-mediated oxidation, hypertrophy, and fibrosis in H9c2 cells and attenuate the inflammation- and oxidation-mediated cardiac injury. In this study, PHL induced significantly inhibitory effect on the expression of pro-inflammatory, hypertrophy, pro-oxidant, and fibrosis cytokines in high glucose-stimulated cardiac H9c2 cells. Furthermore, PHL decreased the levels of serum lactate dehydrogenase, aspartate aminotransferase, and creatine kinase-MB, and attenuated the progress in the fibrosis, oxidative stress, and pathological parameters via Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor E2-related factor 2 (Nrf2) pathway in diabetic mice. In additional, molecular modeling and immunoblotting results confirmed that PHL might obstruct the interaction between Nrf2 and Keap1 through direct binding Keap1, and promoting Nrf2 expression. These results provided evidence that PHL could suppress high glucose-induced cardiomyocyte oxidation and fibrosis injury, and that targeting Keap1/Nrf2 may provide a novel therapeutic strategy for human DCM in the future

Aberrant intrinsic functional brain topology in methamphetamine-dependent individuals after six-months of abstinence

Our aim was to explore the aberrant intrinsic functional topology in methamphetamine-dependent individuals after six months of abstinence using resting-state functional magnetic imaging (rs-fMRI). Eleven methamphetamines (MA) abstainers who have abstained for six months and eleven healthy controls (HC) were recruited for rs-fMRI examination. The graph theory and functional connectivity (FC) analysis were employed to investigate the aberrant intrinsic functional brain topology between the two groups at multiple levels. Compared with the HC group, the characteristic shortest path length (${L}_{p}$) showed a significant decrease at the global level, while the global efficiency (${E}_{glob}$) and local efficiency (${E}_{loc}$) showed an increase considerably. After FDR correction, we found significant group differences in nodal degree and nodal efficiency at the regional level in the ventral attentional network (VAN), dorsal attentional network (DAN), somatosensory network (SMN), visual network (VN) and default mode network (DMN). In addition, the NBS method presented the aberrations in edge-based FC, including frontoparietal network (FPN), subcortical network (SCN), VAN, DAN, SMN, VN and DMN. Moreover, the FC of large-scale functional brain networks revealed a decrease within the VN and SCN and between the networks. These findings suggest that some functions, e.g., visual processing skills, object recognition and memory, may not fully recover after six months of withdrawal. This leads to the possibility of relapse behavior when confronted with MA-related cues, which may contribute to explaining the relapse mechanism. We also provide an imaging basis for revealing the neural mechanism of MA-dependency after six months of abstinence